%Aigaion2 BibTeX export from Idiap Publications
%Thursday 21 November 2024 02:24:28 PM

@INPROCEEDINGS{Chan_ISBI16_2016,
         author = {Chan, Kevin G. and Liebling, Michael},
       keywords = {deconvolution, filtered backprojection, optical microscopy, Optical projection tomography, point-spread-function, tomography.},
       projects = {Idiap},
          month = apr,
          title = {A Point-Spread-Function-Aware Filtered Backprojection Algorithm for Focal-Plane-Scanning Optical Projection Tomography},
      booktitle = {2016 IEEE International Symposium on Biomedical Imaging},
           year = {2016},
       abstract = {Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in blur in the reconstructed image. In this paper, we present a modified filtered backprojection method suitable for focal-plane-scanning optical projection tomography (FPSOPT), where each projection is obtained by scanning through focal planes during collection. We show that FPS-OPT has an exact inversion formula akin to a filtered backprojection, but that incorporates the system’s point-spread-function to recover a deblurred 3D volume. With simulations, we demonstrate that FPS-OPT permits the use of high numerical aperture objectives that lead to more accurate images. We further illustrate the technique on experimentally acquired data from a fluorescently-labeled zebrafish larva, which shows that our approach reduces out-of-focus blur.}
}