%Aigaion2 BibTeX export from Idiap Publications %Saturday 21 December 2024 05:21:22 PM @INPROCEEDINGS{Jaques_ISBI_2019, author = {Jaques, Christian and Bapst-Wicht, Linda and Schorderet, Daniel F. and Liebling, Michael}, projects = {Idiap, COMETS-M, PLATFORM_MMD}, month = apr, title = {Multi-Spectral Widefield Microscopy of the Beating Heart through Post-Acquisition Synchronization and Unmixing}, booktitle = {2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019)}, year = {2019}, pages = {1382-1385}, location = {Venice, Italy}, issn = {1945-8452}, doi = {10.1109/ISBI.2019.8759472}, abstract = {Multi-spectral imaging allows distinguishing biological structures. For cardiac microscopy, available devices are either too slow or require illumination intensities that are detrimental to the sample. We present a method for spectral super-resolution imaging of samples whose motion is quasi-periodic by sequentially acquiring movies in wavelength ranges with filters of overlapping bands. Following an initial calibration procedure, we synchronize and unmix the movies to produce multi-spectral sequences. We characterized our approach to retrieve the transmittance of a colored microscopic target whose motion we controlled, observing measurements within of 10\% that of a reference spectrometer. We further illustrate our approach to observe the beating embryonic zebrafish heart, demonstrating new possibilities for studying its development}, pdf = {https://publications.idiap.ch/attachments/papers/2019/Jaques_ISBI_2019.pdf} }