Simultaneous temporal superresolution and denoising for cardiac fluorescence microscopy
| Type of publication: | Journal paper |
| Citation: | Chan_IEEETRANS.COMPUT.IMAG._2016 |
| Publication status: | Accepted |
| Journal: | IEEE Transactions on Computational Imaging |
| Year: | 2016 |
| Note: | in press |
| ISSN: | 2333-9403 |
| URL: | http://ieeexplore.ieee.org/xpl... |
| DOI: | 10.1109/TCI.2016.2579606 |
| Abstract: | Due to low light emission of fluorescent samples, live fluorescence microscopy imposes a tradeoff between spatiotemporal resolution and signal-to-noise ratio. This can result in images and videos containing motion blur or Poisson-type shot noise, depending on the settings used during acquisition. Here, we propose an algorithm to simultaneously denoise and temporally super-resolve movies of repeating microscopic processes that is compatible with any conventional microscopy setup that can achieve imaging at a rate of at least twice that of the fundamental frequency of the process (above 4 frames per second for a 2 Hz process). Our method combines low temporal resolution frames from multiple cycles of a repeating process to reconstruct a denoised, higher temporal resolution image sequence which is the solution to a linear program that maximizes the consistency of the reconstruction with the measurements, under a regularization constraint. This paper describes, in particular, a parallelizable superresolution reconstruction algorithm and demonstrates its application to live cardiac fluorescence microscopy. Using our method, we experimentally show temporal resolution improvement by a factor of 1.6, resulting in a visible reduction of motion blur in both on-sample and off-sample frames. |
| Keywords: | Fluorescence Microscopy, image denoising, image reconstruction, motion blur, Temporal superresolution |
| Projects |
Idiap |
| Authors | |
| Added by: | [UNK] |
| Total mark: | 0 |
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