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 [BibTeX] [Marc21]
Temporal Super-Resolution Microscopy Using a Hue-Encoded Shutter
Type of publication: Journal paper
Citation: Jaques_OSABOE_2019
Publication status: Accepted
Journal: Optical Society of America Biomedical Optics Express
Volume: 10
Number: 09
Year: 2019
Month: September
Pages: 4727-4741
URL: https://www.osapublishing.org/...
DOI: doi.org/10.1364/BOE.10.004727
Abstract: Limited time-resolution in microscopy is an obstacle to many biological studies. Despite recent advances in hardware, digital cameras have limited operation modes that constrain frame-rate, integration time, and color sensing patterns. In this paper, we propose an approach to extend the temporal resolution of a conventional digital color camera by leveraging a multi-color illumination source. Our method allows imaging single-hue objects at increased frame-rate by trading spectral for temporal information (while retaining the ability to measure base hue). It also allows rapid switching to standard RGB acquisition. We evaluated the feasibility and performance of our method via experiments with mobile resolution targets. We observed a time-resolution increase by a factor 2.8 with a three-fold increase in temporal sampling rate. We further illustrate the use of our method to image the beating heart of a zebrafish larva, allowing the display of color or fast grayscale images. Our method is particularly well-suited to extend the capabilities of imaging systems where the flexibility of rapidly switching between high frame rate and color imaging are necessary.
Projects Idiap
Authors Jaques, Christian
Pignat, Emmanuel
Calinon, Sylvain
Liebling, Michael
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Total mark: 0