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 [BibTeX] [Marc21]
Unequivocal cardiac phase sorting from alternating ramp- and pulse-illuminated microscopy image sequences
Type of publication: Conference paper
Citation: Mariani_ISBI21_2021
Publication status: Published
Booktitle: 2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI)
Year: 2021
Month: April
Pages: 868-872
URL: https://doi.org/10.1109/ISBI48...
DOI: 10.1109/ISBI48211.2021.9433858
Abstract: In vivo microscopy is an important tool to study developing organs such as the heart of the zebrafish embryo but is often limited by slow image frame acquisition speed. While collections of still images of the beating heart at arbitrary phases can be sorted to obtain a virtual heartbeat, the presence of identical heart configurations at two or more heartbeat phases can derail this approach. Here, we propose a dual illumination method to encode movement in alternate frames to disambiguate heartbeat phases in the still frames. We propose to alternately acquire images with a ramp and pulse illumination then sort all successive image pairs based on the ramp-illuminated data but use the pulse-illuminated images for display and analysis. We characterized our method on synthetic data, and show its applicability on experimental data and found that an exposure time of about 7% of the heartbeat or more is necessary to encode the movement reliably in a single heartbeat with a single redundant node. Our method opens the possibility to use sorting algorithms without prior information on the phase, even when the movement presents redundant frames.
Keywords: Asymmetry, Heartbeat, live microscopy, Periodicity, Structured illumination, Zebrafish
Projects Idiap
Authors Mariani, Olivia
Marelli, Fran├žois
Jaques, Christian
Ernst, Alexander
Liebling, Michael
Added by: [UNK]
Total mark: 0
  • Mariani_ISBI21_2021.pdf