logo Idiap Research Institute        
 [BibTeX] [Marc21]
Simultaneous temporal superresolution and denoising for cardiac fluorescence microscopy
Type of publication: Journal paper
Citation: Chan_IEEETRANS.COMPUT.IMAG._2016
Publication status: Accepted
Journal: IEEE Transactions on Computational Imaging
Year: 2016
Note: in press
ISSN: 2333-9403
URL: http://ieeexplore.ieee.org/xpl...
DOI: 10.1109/TCI.2016.2579606
Abstract: Due to low light emission of fluorescent samples, live fluorescence microscopy imposes a tradeoff between spatiotemporal resolution and signal-to-noise ratio. This can result in images and videos containing motion blur or Poisson-type shot noise, depending on the settings used during acquisition. Here, we propose an algorithm to simultaneously denoise and temporally super-resolve movies of repeating microscopic processes that is compatible with any conventional microscopy setup that can achieve imaging at a rate of at least twice that of the fundamental frequency of the process (above 4 frames per second for a 2 Hz process). Our method combines low temporal resolution frames from multiple cycles of a repeating process to reconstruct a denoised, higher temporal resolution image sequence which is the solution to a linear program that maximizes the consistency of the reconstruction with the measurements, under a regularization constraint. This paper describes, in particular, a parallelizable superresolution reconstruction algorithm and demonstrates its application to live cardiac fluorescence microscopy. Using our method, we experimentally show temporal resolution improvement by a factor of 1.6, resulting in a visible reduction of motion blur in both on-sample and off-sample frames.
Keywords: Fluorescence Microscopy, image denoising, image reconstruction, motion blur, Temporal superresolution
Projects Idiap
Authors Chan, Kevin G.
Streichan, Sebastian J.
Trinh, Le A.
Liebling, Michael
Added by: [UNK]
Total mark: 0
  • Chan_IEEETRANS.COMPUT.IMAG._2016.pdf
       (This article has been accepted for publication in a future issue of this journal, but has not been fully edited. Content may change prior to final publication. http://dx.doi.org/10.1109/TCI.2016.2579606)