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 [BibTeX] [Marc21]
Multi-Spectral Widefield Microscopy of the Beating Heart through Post-Acquisition Synchronization and Unmixing
Type of publication: Conference paper
Citation: Jaques_ISBI_2019
Publication status: Accepted
Booktitle: 2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019)
Year: 2019
Month: April
Pages: 1382-1385
Location: Venice, Italy
ISSN: 1945-8452
DOI: 10.1109/ISBI.2019.8759472
Abstract: Multi-spectral imaging allows distinguishing biological structures. For cardiac microscopy, available devices are either too slow or require illumination intensities that are detrimental to the sample. We present a method for spectral super-resolution imaging of samples whose motion is quasi-periodic by sequentially acquiring movies in wavelength ranges with filters of overlapping bands. Following an initial calibration procedure, we synchronize and unmix the movies to produce multi-spectral sequences. We characterized our approach to retrieve the transmittance of a colored microscopic target whose motion we controlled, observing measurements within of 10% that of a reference spectrometer. We further illustrate our approach to observe the beating embryonic zebrafish heart, demonstrating new possibilities for studying its development
Projects Idiap
Authors Jaques, Christian
Bapst-Wicht, Linda
Schorderet, Daniel F.
Liebling, Michael
Added by: [UNK]
Total mark: 0
  • Jaques_ISBI_2019.pdf
       (preprint submission to ISBI 2019)