Generalized temporal sampling with active illumination in optical microscopy
| Type of publication: | Conference paper |
| Citation: | Jaques_WAVELETSANDSPARSITYXVIII_2019 |
| Publication status: | Accepted |
| Booktitle: | Proceeding of the SPIE Conference Optics and Photonics, Wavelets and Sparsity XVIII |
| Volume: | 11138 |
| Year: | 2019 |
| Month: | August |
| Publisher: | SPIE |
| Location: | San Diego, California, United States |
| Organization: | SPIE |
| Abstract: | Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overlapping inner-products and because only positive signals (intensities) can be measured by cameras. We present a method to collect videos of monochromatic objects by projecting the incoming signal at each pixel in a temporal B-spline space of degree 0, 1, or 2 by using a conventional RGB camera and a modulated three-color light source for illumination. Specifically, we solve the basis function overlap problem by multiplexing the acquisition in different color ranges and use B-spline pieces (which are positive) as projection kernels of a biorthogonal projection-expansion bases pair. The steps to recover signal samples include spectral unmixing and inverse filtering. Reconstructions we obtained from simulated and experimentally-acquired microscopy data demonstrate the feasibility of our approach. |
| Keywords: | B-splines, computational imaging, Generalized sampling, microscopy, spectral unmixing, wavelets |
| Projects: |
Idiap COMETS-M PLATFORM_MMD COMPBIO |
| Authors: | |
| Added by: | [UNK] |
| Total mark: | 0 |
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