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 [BibTeX] [Marc21]
Generalized temporal sampling with active illumination in optical microscopy
Type of publication: Conference paper
Publication status: Accepted
Booktitle: Proceeding of the SPIE Conference Optics and Photonics, Wavelets and Sparsity XVIII
Volume: 11138
Year: 2019
Month: August
Publisher: SPIE
Location: San Diego, California, United States
Organization: SPIE
Abstract: Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overlapping inner-products and because only positive signals (intensities) can be measured by cameras. We present a method to collect videos of monochromatic objects by projecting the incoming signal at each pixel in a temporal B-spline space of degree 0, 1, or 2 by using a conventional RGB camera and a modulated three-color light source for illumination. Specifically, we solve the basis function overlap problem by multiplexing the acquisition in different color ranges and use B-spline pieces (which are positive) as projection kernels of a biorthogonal projection-expansion bases pair. The steps to recover signal samples include spectral unmixing and inverse filtering. Reconstructions we obtained from simulated and experimentally-acquired microscopy data demonstrate the feasibility of our approach.
Keywords: B-splines, computational imaging, Generalized sampling, microscopy, spectral unmixing, wavelets
Projects Idiap
Authors Jaques, Christian
Liebling, Michael
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