A Point-Spread-Function-Aware Filtered Backprojection Algorithm for Focal-Plane-Scanning Optical Projection Tomography
| Type of publication: | Conference paper |
| Citation: | Chan_ISBI16_2016 |
| Publication status: | Accepted |
| Booktitle: | 2016 IEEE International Symposium on Biomedical Imaging |
| Year: | 2016 |
| Month: | April |
| Abstract: | Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in blur in the reconstructed image. In this paper, we present a modified filtered backprojection method suitable for focal-plane-scanning optical projection tomography (FPSOPT), where each projection is obtained by scanning through focal planes during collection. We show that FPS-OPT has an exact inversion formula akin to a filtered backprojection, but that incorporates the system’s point-spread-function to recover a deblurred 3D volume. With simulations, we demonstrate that FPS-OPT permits the use of high numerical aperture objectives that lead to more accurate images. We further illustrate the technique on experimentally acquired data from a fluorescently-labeled zebrafish larva, which shows that our approach reduces out-of-focus blur. |
| Keywords: | deconvolution, filtered backprojection, optical microscopy, Optical projection tomography, point-spread-function, tomography. |
| Projects |
Idiap |
| Authors | |
| Added by: | [UNK] |
| Total mark: | 0 |
|
Attachments
|
|
|
Notes
|
|
|
|
|